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doi: 10.1111/j.1600-6143.2007.01927.x 109 PeaJRPinneyJRAyalaPDesaiTAGoldspinkPH

Primers were designed to fit in resulting vector, pJB-HTS upstream of the hexa-histadine tag (5/phos/CCATGGGCAGCAGCCATCATCAT), and downstream (AGCTGGAATTCCTAGTTATTGCTCAGCGGTGGC) (Integrated DNA technologies) of the spacer yielding a fragment containing a phosphorylated 5 end, an initiation codon, a hexa-histadine tag, a thrombin cleavable sequence, a spacer, a termination codon, and an EcoRI site

CJC-1295 is a GHRH analog (it tells the pituitary to make GH), while ipamorelin is a growth-hormone secretagogue (it triggers the release of stored GH)