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Then cDNA was synthesized using SuperScript TM IV VILO TM Master Mix (Invitrogen, Waltham, MA, United States) and diluted 101 by DNase/RNase-Free Distilled Water (Invitrogen)

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The key components work together to support weight management: Methionine (15mg): An essential amino acid that helps metabolize fat and supports the digestive system Inositol (50mg): Regulates insulin and glucose metabolism while supporting cellular nutrient transfer Choline (100mg): Assists in controlling cholesterol levels and maintaining healthy cell membranes Vitamin B12 (1000mcg): Supports energy metabolism and red blood cell production These nutrients play crucial roles in the body's fat utilization processes, enhancing the liver and gallbladder's function by decreasing fat deposits and accelerating fat metabolism