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Reverse transcription was done using the RevertAid first-strand cDNA synthesis Kit (Thermo Fisher Scientific, USA) and quantitative PCR was performed using the SsoAdvanced Universal SYBR Green Supermix (Bio-Rad, USA) on a Bio-Rad CFX96-Real-Time PCR Detection System

The currently available studies support the concept that increased oxidative stress (OS) and deficient antioxidant protection is implicated in the etiology of recurrent pregnancy loss (RPL) but underlying mechanisms through which OS affects pregnancy outcome remains largely indefinable

The combination treatment significantly improved both ovulation rates and cumulative pregnancy rates compared to clomiphene citrate alone

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