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This work was supported by the National 14th Five-Year Plan Key Research and Development Program (2024YFD1300204, 2023YFD1301705), high-end foreign experts from the Ministry of Science and Technology of China (G2023014066L), and the Priority Academic Program Development of Jiangsu Higher Education Institution (PAPD), P.R

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in contrast, Prevotella melaninogenica, Streptococcus anginosus, and Propionibacterium acnes numbers were increased in tumor samples compared to the normal and peritumoral samples (Liu et al., 2019)

Subsequently, cells were continuously screened with medium containing 2 g/ml puromycin and passaged for three generations to maintain stable expression, which were then preserved