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In AD, calcium influx becomes dysregulated, leading to elevated intracellular calcium levels

Folic acid supplementation is recommended during pregnancy due to its prophylactic role, but its influence on women undergoing ART is not as widely recognized

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Therefore, understanding what factors can negatively modulate tight junction permeability and how pharmacological approaches such as LA act to protect or restore the tight junction barrier before or after injury is critical for developing preventative or therapeutic approaches