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To further substantiate these results, we measured the expression of hexokinase 1 (HK1), a rate limiting enzyme of glycolysis

Inject the water against the inside wall of the vial never directly onto the lyophilized powder, which causes foaming and surface denaturation

Although energy metabolism status can also regulate intracellular ROS levels (e.g., glucose deprivation leads to a marked increase in cellular ROS) [53,54,55,56], our study demonstrated that supplementation with energy substrates such as sodium pyruvate in GGCT-knockdown cell models did not induce significant alterations in intracellular ROS levels

This lack of variants is common in essential biochemical pathways, probably because variants would be too detrimental to be compatible with life